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IFN-gamma Polyklonaler Antikörper

IFN-gamma Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15365-1-PBS

Synonyme

IFNG, IFG, IFN gamma, IFN γ, IFNγ

Filter:
  • Western Blot (WB) analysis of various lysates using IFN-gamma Polyclonal antibody (15365-1-AP)

    WB analysis using 15365-1-AP (same clone as 15365-1-PBS)

    PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15365-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using IFN-gamma Polyclonal antibody (15365-1-AP)

    WB analysis of Recombinant protein using 15365-1-AP (same clone as 15365-1-PBS)

    Recombinant human IFN gamma protein (cat.NO. HZ-1301) were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15365-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IFN-gamma Polyclonal antibody (15365-1-AP)

    IHC staining of human tonsillitis using 15365-1-AP (same clone as 15365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15365-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15365-1-PBS bindet in WB, IHC, ELISA IFN-gamma und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IFN-gamma fusion protein Ag8321
Vollständiger Name IFN gamma
Berechnetes Molekulargewicht 166 aa, 19 kDa
Beobachtetes Molekulargewicht22 kDa
GenBank-ZugangsnummerBC070256
Gene symbol IFNG
Gene ID (NCBI) 3458
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.