NeutraKine® IFN-gamma Monoklonaler Antikörper

NeutraKine® IFN-gamma Monoklonal Antikörper für Neutralization, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Neutralization, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1E10G7

Kat-Nr. : 69007-1-Ig

Synonyme

NeutraKine® IFN Gamma, IFNG, 1E10G7, IFG, IFN gamma

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  Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

  Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (2ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL.

• 

  Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

  Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

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  ELISA experiment of Recombinant protein using 69007-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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• Neutralization: HT-29 cell

Produktinformation

69007-1-Ig bindet in Neutralization, ELISA, Cell treatment NeutraKine® IFN-gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human Humankine IFN-gamma protein HZ-1301
• Vollständiger Name: IFN gamma
• Gene symbol: IFNG
• Gene ID (NCBI): 3458
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: Sterile PBS
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.

Publikationen

Species	Application	Title
	Neutralization	Cell Mol Gastroenterol Hepatol; Fatty Acid Oxidation Promotes Apoptotic Resistance and Proinflammatory Phenotype of CD4+ Tissue-resident Memory T cells in Crohn's Disease; Authors - Guanzhan Liang
human	Cell treatment	Nat Commun; T-bet+CD8+ T cells govern anti-PD-1 responses in microsatellite-stable gastric cancers; Authors - Shiying Tang