IFNAR1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

IFNAR1 Rekombinant Antikörper für WB, FC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230549C8

Kat-Nr. : 83002-4-RR

Synonyme

230549C8, AVP, CRF2 1, CRF2-1, Cytokine receptor class-II member 1

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  WB analysis of HeLa using 83002-4-RR

  WB result of IFNAR1 antibody (83002-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFNAR1 transfected HeLa cells.

• 

  WB analysis using 83002-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83002-4-RR

  1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  FC experiment of K562 using 83002-4-RR

  1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 83002-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen, K562

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83002-4-RR bindet in WB, FC, ELISA IFNAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFNAR1 fusion protein Eg0156
• Vollständiger Name: interferon (alpha, beta and omega) receptor 1
• Berechnetes Molekulargewicht: 557 aa, 64 kDa
• Beobachtetes Molekulargewicht: 80-140 kDa
• GenBank-Zugangsnummer: BC021825
• Gene symbol: IFNAR1
• Gene ID (NCBI): 3454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.