IFN-gamma Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFN-gamma Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(132)

Kat-Nr. : 15365-1-AP

Synonyme

IFNG, IFG, IFN gamma, IFN γ, IFNγ

---

• 

  WB analysis using 15365-1-AP

  PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 15365-1-AP

  Recombinant human IFN gamma protein (cat.NO. HZ-1301) were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 15365-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells, Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 56 publications below
• IHC: See 46 publications below
• IF: See 35 publications below
• ELISA: See 1 publications below

Produktinformation

15365-1-AP bindet in WB, IHC, IF, ELISA IFN-gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFN-gamma fusion protein Ag8321
• Vollständiger Name: IFN gamma
• Berechnetes Molekulargewicht: 166 aa, 19 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC070256
• Gene symbol: IFNG
• Gene ID (NCBI): 3458
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Publikationen

Species	Application	Title
human	IF	Nat Immunol; Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.; Authors - Di Huang
human	IF	Adv Sci (Weinh); Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy; Authors - Xiaoling Wu
	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
human	IF	Gastroenterology; Interferon-γ-stimulated genes, but not USP18, are expressed in livers of patients with acute hepatitis C.; Authors - Michael T Dill
	WB,IHC	eBioMedicine; Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma; Authors - Tingyu Wen
	WB	J Control Release; Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization; Authors - Tao Bo