IFNGR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFNGR1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10808-1-AP

Synonyme

CD119, CDw119, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10808-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10808-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10808-1-AP

  IP Result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:500) with HepG2 cells lysate 1800ug.

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  IP experiment of HepG2 using 10808-1-AP

  IP Result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:300) with HepG2 cells lysate 3000ug.

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  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 10808-1-AP

  1X10^6 HepG2 cells were stained with .2ug IFNGR1 antibody (10808-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10808-1-AP bindet in WB, IP, IHC, IF, FC, ELISA IFNGR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFNGR1 fusion protein Ag1114
• Vollständiger Name: interferon gamma receptor 1
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC005333
• Gene symbol: IFNGR1
• Gene ID (NCBI): 3459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Publikationen

Species	Application	Title
mouse	IHC	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Dev Cell; Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells.; Authors - Elena Parmigiani
human,mouse	WB	Oncogene; Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia; Authors - Xiaoling Xie ; KD Validated
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ
mouse	WB	J Biol Chem; PMID: 28174303; Authors - Barbara Kroczynska

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