IFNGR1/CD119 Polyklonaler Antikörper

IFNGR1/CD119 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31951-1-AP

Synonyme

CD119, IFN-gamma R1, IFN-gamma receptor 1, IFN-gamma-R1, IFN-gamma-R-alpha

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  WB analysis using 31951-1-AP

  EL-4 cells were subjected to SDS PAGE followed by western blot with 31951-1-AP (IFN-gamma R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of EL-4 using 31951-1-AP

  IP result of anti-IFNGR1 (IP:31951-1-AP, 4ug; Detection:31951-1-AP 1:600) with EL-4 cells lysate 2080 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EL-4 cells
• Erfolgreiche IP: EL-4 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31951-1-AP bindet in WB, IP, ELISA IFNGR1/CD119 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFNGR1/CD119 fusion protein Eg1536
• Vollständiger Name: interferon gamma receptor 1
• Berechnetes Molekulargewicht: 52kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: NM_010511.3
• Gene symbol: Ifngr1
• Gene ID (NCBI): 15979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)