IFNGR1 Monoklonaler Antikörper

IFNGR1 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3A4

Kat-Nr. : 66704-1-Ig

Synonyme

CD119, CDw119, FLJ45734, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66704-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66704-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66704-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66704-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66704-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66704-1-Ig (IFNGR1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66704-1-Ig bindet in WB,ELISA IFNGR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFNGR1 fusion protein Ag27085
• Vollständiger Name: interferon gamma receptor 1
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC005333
• Gene symbol: IFNGR1
• Gene ID (NCBI): 3459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)