IFT140 Rekombinanter Antikörper

IFT140 Rekombinant Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

230231F7

Kat-Nr. : 82947-1-RR

Synonyme

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

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  WB analysis of HeLa using 82947-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 82947-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 82947-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-1-RR, Clone: 230231F7 ) at dilution of 1:120 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: Starvation treated hTERT-RPE1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82947-1-RR bindet in WB, IF, ELISA IFT140 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFT140 fusion protein Ag11119
• Vollständiger Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 1462 aa, 165 kDa
• Beobachtetes Molekulargewicht: 150-165 kDa
• GenBank-Zugangsnummer: BC035577
• Gene symbol: IFT140
• Gene ID (NCBI): 9742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)