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IFT140 Rekombinanter Antikörper

IFT140 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230231D5

Kat-Nr. : 82947-7-PBS

Synonyme

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

Filter:
  • Western Blot (WB) analysis of HeLa cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT140 Recombinant antibody (82947-7-RR)

    IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82947-7-PBS

    Affinity and Kinetic Characterization of 82947-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82947-7-PBS bindet in WB, IF/ICC, ELISA IFT140 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IFT140 fusion protein Ag11119
Vollständiger Name intraflagellar transport 140 homolog (Chlamydomonas)
Berechnetes Molekulargewicht 1462 aa, 165 kDa
GenBank-ZugangsnummerBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.