IFT140 Polyklonaler Antikörper

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• KD/KO Validated

IFT140 Polyklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-17460

Synonyme

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

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  IF Staining of mouse testis using CL594-17460

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

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  IF Staining of mouse testis using CL594-17460

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

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  IF Staining of hTERT-RPE1 using CL594-17460

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-17460 bindet in IF/ICC, IF-P IFT140 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT140 fusion protein Ag11119
• Vollständiger Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 1462 aa, 165 kDa
• GenBank-Zugangsnummer: BC035577
• Gene symbol: IFT140
• Gene ID (NCBI): 9742
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)