IFT172 Rekombinanter Antikörper

IFT172 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250711E6

Kat-Nr. : 86109-1-PBS

Synonyme

IFT, KIAA1179, NPHP17, osm 1, SLB

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  WB analysis of mouse testis using 86109-1-RR (same clone as 86109-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 86109-1-RR (same clone as 86109-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 86109-1-RR (same clone as 86109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT172 antibody (86109-1-RR, Clone: 250711E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86109-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86109-1-RR against Human IFT172 were performed. The affinity constant is 2.02 nM.

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Geprüfte Anwendungen

Produktinformation

86109-1-PBS bindet in WB, IF/ICC, Indirect ELISA IFT172 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFT172 fusion protein Ag28921
• Vollständiger Name: intraflagellar transport 172 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 1749 aa, 198 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC137126
• Gene symbol: IFT172
• Gene ID (NCBI): 26160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.