IFT57 Polyklonaler Antikörper

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IFT57 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11083-1-PBS

Synonyme

Dermal papilla-derived protein 8, DERP8, ESRRBL1, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein

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  WB analysis of mouse retina using 11083-1-AP (same clone as 11083-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

  WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  WB analysis using 11083-1-AP (same clone as 11083-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

  IP result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 11083-1-AP (same clone as 11083-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT57 antibody (11083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11083-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA IFT57 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT57 fusion protein Ag1566
• Vollständiger Name: intraflagellar transport 57 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC011899
• Gene symbol: IFT57
• Gene ID (NCBI): 55081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)