IFT74 Polyklonaler Antikörper

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• KD/KO Validated

IFT74 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 27334-1-AP

Synonyme

CCDC2, CMG 1, CMG1, IFT74

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 27334-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27334-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 27334-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

27334-1-AP bindet in WB, IP, IF, CoIP, ELISA IFT74 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT74 fusion protein Ag26385
• Vollständiger Name: intraflagellar transport 74 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 600 aa, 69 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC107742
• Gene symbol: IFT74
• Gene ID (NCBI): 80173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Genet Med; Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.; Authors - Minna Luo ; KD Validated
human	WB	Pulm Circ; Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia; Authors - Saejeong Park
human,mouse	IF	Hum Reprod; Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility; Authors - Qi Li
human	WB,IP	Hum Mol Genet; Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations; Authors - Mahmoud R Fassad
human	WB	EMBO J; The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport; Authors - Niels Boegholm ; KO Validated
mouse	WB,CoIP	EMBO Mol Med; IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect; Authors - Jing Dai