IFT80 Polyklonaler Antikörper

IFT80 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25230-1-AP

Synonyme

ATD2, IFT80, KIAA1374, WDR56

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  WB analysis of NIH/3T3 using 25230-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 25230-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25230-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maushirngewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

25230-1-AP bindet in WB, IF, ELISA IFT80 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT80 fusion protein Ag18650
• Vollständiger Name: intraflagellar transport 80 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 777 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88-90 kDa
• GenBank-Zugangsnummer: BC101494
• Gene symbol: IFT80
• Gene ID (NCBI): 57560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	EMBO Rep; Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery.; Authors - Huijie Zhao
mouse	WB	Mol Neurobiol; Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure; Authors - Xiuwei Wang
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
mouse	WB	Life (Basel); The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study; Authors - Hyeran Helen Jeon
mouse	IF	Cell Insight; Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling; Authors - Gongsheng Yuan