IFT81 Monoklonaler Antikörper

IFT81 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5A3

Kat-Nr. : 67987-1-Ig

Synonyme

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Galerie der Validierungsdaten

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  WB analysis using 67987-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 67987-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 67987-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (67987-1-Ig, Clone: 1D5A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, hTERT-RPE1-Zellen, humanes Hodengewebe, Kaninchenhirngewebe, Maushodengewebe, MDCK-Zellen, NCCIT-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67987-1-Ig bindet in WB, IF, ELISA IFT81 und zeigt Reaktivität mit human, Hund, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFT81 fusion protein Ag31629
• Vollständiger Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 676 aa, 80 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC029349
• Gene symbol: IFT81
• Gene ID (NCBI): 28981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.