IFT88 Monoklonaler Antikörper

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• KD/KO Validated

IFT88 Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A4G5

Kat-Nr. : 60227-1-PBS

Synonyme

4A4G5, Intraflagellar transport protein 88 homolog, TG737, TTC10

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  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 60227-1-Ig (same clone as 60227-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

  IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60227-1-PBS bindet in WB, IHC, IP, Indirect ELISA IFT88 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFT88 fusion protein Ag4980
• Vollständiger Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 88-95 kDa
• GenBank-Zugangsnummer: BC030776
• Gene symbol: IFT88
• Gene ID (NCBI): 8100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.