IGF1 Rekombinanter Antikörper

IGF1 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241890G6

Kat-Nr. : 84782-5-RR

Synonyme

241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin

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  WB analysis of rat plasma using 84782-5-RR

  rat plasma were subjected to SDS PAGE followed by western blot with 84782-5-RR (IGF-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 84782-5-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 84782-5-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Transfected HEK-293T using 84782-5-RR

  1x10^6 HEK-293T cells or IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug IGF1 Recombinant antibody (84782-5-RR, Clone:241890G6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84782-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84782-5-RR against Rat IGF1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rat plasma
• Erfolgreiche Detektion in IHC: Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84782-5-RR bindet in WB, IHC, FC (Intra), ELISA IGF1 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IGF1 fusion protein Eg1976
• Vollständiger Name: insulin-like growth factor 1
• Berechnetes Molekulargewicht: 14kd
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: AAA41386
• Gene symbol: Igf1
• Gene ID (NCBI): 24482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.