IGF1R/CD221 Monoklonaler Antikörper

IGF1R/CD221 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C6A11

Kat-Nr. : 66283-1-PBS

Synonyme

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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  WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66283-1-Ig (same clone as 66283-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66283-1-Ig (same clone as 66283-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66283-1-Ig (same clone as 66283-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 66283-1-Ig (same clone as 66283-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66283-1-PBS bindet in WB, IHC, Indirect ELISA IGF1R/CD221 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IGF I receptor
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: NM_000875
• Gene symbol: IGF1R
• Gene ID (NCBI): 3480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.