IGF1R/CD221 Rekombinanter Antikörper

IGF1R/CD221 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241679A6

Kat-Nr. : 98144-1-RR

Synonyme

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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• 

  FC experiment of human peripheral blood granulocytes using 98144-1-RR

  1x10^6 human peripheral blood granulocytes were surface stained with 0.25 ug Anti-Human IGF1R/CD221 Rabbit Recombinant Antibody (98144-1-RR, Clone: 241679A6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98144-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98144-1-RR against Human IGF1R/CD221 were performed. The affinity constant is 83.6 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Granulozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98144-1-RR bindet in FC IGF1R/CD221 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: IGF I receptor
• Berechnetes Molekulargewicht: 1367 aa, 155 kDa
• GenBank-Zugangsnummer: BC113612
• Gene symbol: IGF1R
• Gene ID (NCBI): 3480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.