IGF2 Rekombinanter Antikörper

IGF2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241746D3

Kat-Nr. : 84643-1-RR

Synonyme

241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446

---

• 

  WB analysis of HuH-7 using 84643-1-RR

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84643-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84643-1-RR bindet in WB, IHC, ELISA IGF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IGF2 fusion protein HZ-1161
• Vollständiger Name: insulin-like growth factor 2 (somatomedin A)
• Beobachtetes Molekulargewicht: 20 kDa
• Gene symbol: IGF2
• Gene ID (NCBI): 3481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.