IGF2BP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGF2BP1 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-22803

Synonyme

IMP 1, IGF-II mRNA-binding protein 1, IGF2BP 1, IGF2 mRNA-binding protein 1, CRD-BP

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  IF Staining of A375 using CL488-22803

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CoraLite® Plus 488 IGF2BP1 antibody (CL488-22803) at dilution of 1:200, CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-22803 bindet in IF/ICC IGF2BP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGF2BP1 fusion protein Ag18859
• Vollständiger Name: insulin-like growth factor 2 mRNA binding protein 1
• Berechnetes Molekulargewicht: 577 aa, 63 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC156957
• Gene symbol: IGF2BP1
• Gene ID (NCBI): 10642
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.