IGF2BP2 Polyklonaler Antikörper

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• KD/KO Validated

IGF2BP2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11601-1-PBS

Synonyme

IGF2 mRNA-binding protein 2, IGF-II mRNA-binding protein 2, IMP2, IMP-2, insulin like growth factor 2 mRNA binding protein 2

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  WB analysis using 11601-1-AP (same clone as 11601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11601-1-AP (same clone as 11601-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 11601-1-AP (same clone as 11601-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11601-1-AP (same clone as 11601-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11601-1-AP (same clone as 11601-1-PBS)

  IP result of anti-IGF2BP2 (IP:11601-1-AP, 3ug; Detection:11601-1-AP 1:500) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11601-1-AP (IGF2BP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11601-1-AP (IGF2BP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11601-1-AP (same clone as 11601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11601-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11601-1-PBS bindet in WB, IHC, IP, Indirect ELISA IGF2BP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGF2BP2 fusion protein Ag2142
• Vollständiger Name: insulin-like growth factor 2 mRNA binding protein 2
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC021290
• Gene symbol: IGF2BP2
• Gene ID (NCBI): 10644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).