IGF2BP2 Rekombinanter Antikörper

IGF2BP2 Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6L8

Kat-Nr. : 82757-2-PBS

Synonyme

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

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  WB analysis using 82757-2-RR (same clone as 82757-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 82757-2-RR (same clone as 82757-2-PBS)

  IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82757-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82757-2-PBS bindet in WB, IHC, IP, Indirect ELISA IGF2BP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IGF2BP2 fusion protein Ag2142
• Vollständiger Name: insulin-like growth factor 2 mRNA binding protein 2
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC021290
• Gene symbol: IGF2BP2
• Gene ID (NCBI): 10644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).