IGF2R-Specific Polyklonaler Antikörper

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• KD/KO Validated

IGF2R-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20253-1-PBS

Synonyme

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222

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  WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

  HepG2 cells lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

  WB result of IGF2R-Specific antibody (20253-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2R-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 20253-1-AP (same clone as 20253-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

  IP result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IGF2R-Specific antibody (20253-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IGF2R-Specific Polyclonal antibody (20253-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20253-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA IGF2R-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin-like growth factor 2 receptor
• Berechnetes Molekulargewicht: 274 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_000876
• Gene symbol: IGF2R
• Gene ID (NCBI): 3482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.