IGFBP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGFBP2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11065-3-AP

Synonyme

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

---

Galerie der Validierungsdaten

• 

  WB analysis of SH-SY5Y using 11065-3-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11065-3-AP (IGFBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11065-3-AP

  IP Result of anti-IGFBP2 (IP:11065-3-AP, 4ug; Detection:11065-3-AP 1:300) with SH-SY5Y cells lysate 1200ug.

• 

  IHC staining of human prostate cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

11065-3-AP bindet in WB, IP, IHC, IF, ELISA IGFBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGFBP2 fusion protein Ag1535
• Vollständiger Name: IGF binding protein 2
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: BC009902
• Gene symbol: IGFBP2
• Gene ID (NCBI): 3485
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INS-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degraded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan ; KD Validated
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim
chicken	WB	Oxid Med Cell Longev; IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.; Authors - Yafan Gong
mouse	WB,IF	Acta Physiol (Oxf); SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice; Authors - Jingjing Zhang
human	IHC,WB	Front Immunol; The cuproptosis-related signature associated with the tumor environment and prognosis of patients with glioma; Authors - Weichen Wang
chicken	WB	Cell Signal; PMID: 28734787; Authors - Jie Yang