IGFBP2 Rekombinanter Antikörper

IGFBP2 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241363D12

Kat-Nr. : 84092-1-PBS

Synonyme

mIGFBP-2, Insulin-like growth factor-binding protein 2, IGFBP-2, IGF-binding protein 2, IBP-2

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  WB analysis using 84092-1-RR (same clone as 84092-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84092-1-RR (IGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84092-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01011-1

  Sandwich ELISA standard curve of MP01011-1, Mouse IGFBP2 Recombinant Matched Antibody Pair - PBS only. 84092-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1196. 84092-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01011-1

  Serum of six mouse was measured. The IGFBP2 concentration of detected samples was determined to be 152.26 ng/mL with a range of 95.84-279.86 ng/mL.

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  Sample test of MP01011-1

  One mouse kidney was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for mouse IGFBP2 and measured 38.18 ng/mL.

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  Cytometric bead array standard curve of MP01011-1

  Cytometric bead array standard curve of MP01011-1, MOUSE IGFBP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84092-1-PBS. Detection antibody: 84092-2-PBS. Standard: Eg1196. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 84092-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84092-1-RR against Mouse IGFBP2 were performed. The affinity constant is 0.571 nM.

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Geprüfte Anwendungen

Produktinformation

84092-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test IGFBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: insulin-like growth factor binding protein 2
• Berechnetes Molekulargewicht: 33kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: NM_008342.3
• Gene symbol: Igfbp2
• Gene ID (NCBI): 16008
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).