IGFBP3 Polyklonaler Antikörper

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• KD/KO Validated

IGFBP3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10189-2-PBS

Synonyme

BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3

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  WB analysis using 10189-2-AP (same clone as 10189-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10189-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IGFBP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGFBP3 fusion protein Ag0242
• Vollständiger Name: insulin-like growth factor binding protein 3
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 40-45 kDa
• GenBank-Zugangsnummer: BC000013
• Gene symbol: IGFBP3
• Gene ID (NCBI): 3486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

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