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IGFBP3 Rekombinanter Antikörper

IGFBP3 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241723B3

Kat-Nr. : 84470-4-PBS

Synonyme

IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53

Filter:
  • Western Blot (WB) analysis of SKOV-3 cells using IGFBP3 Recombinant antibody (84470-4-RR)

    WB analysis of SKOV-3 using 84470-4-RR (same clone as 84470-4-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 84470-4-RR (IGFBP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84470-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84470-4-PBS bindet in WB, Indirect ELISA IGFBP3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FusionProtein
Vollständiger Name insulin-like growth factor binding protein 3
Berechnetes Molekulargewicht32 kDa
Beobachtetes Molekulargewicht 32-37 kDa
GenBank-ZugangsnummerNM_000598.5
Gene symbol IGFBP3
Gene ID (NCBI) 3486
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)