IGFBP6 Monoklonaler Antikörper

IGFBP6 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D12B3

Kat-Nr. : 67567-1-PBS

Synonyme

IGFBP 6, IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6

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  WB analysis of U-87 MG using 67567-1-Ig (same clone as 67567-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 67567-1-Ig (same clone as 67567-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 67567-1-Ig (same clone as 67567-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using IGFBP6 antibody (67567-1-Ig, Clone: 1D12B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67567-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IGFBP6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IGFBP6 fusion protein Ag30050
• Vollständiger Name: insulin-like growth factor binding protein 6
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC011708
• Gene symbol: IGFBP6
• Gene ID (NCBI): 3489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.