IGFBP7 Polyklonaler Antikörper

IGFBP7 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19961-1-AP

Synonyme

FSTL2, IBP 7, IGF binding protein 7, IGFBP 7, IGFBP 7v, IGFBP rP1, IGFBP7, IGFBPRP1, MAC25, MAC25 protein, PGI2 stimulating factor, PSF, TAF, Tumor derived adhesion factor

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Galerie der Validierungsdaten

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  WB analysis using 19961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19961-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 19961-1-AP

  IP Result of anti-IGFBP7 (IP:19961-1-AP, 4ug; Detection:19961-1-AP 1:300) with mouse appendix tissue lysate 6000ug.

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  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen, SKOV-3-Zellen, THP-1-Zellen
• Erfolgreiche IP: Maus-Appendix-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

19961-1-AP bindet in WB, IP, IHC, IF, ELISA IGFBP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin-like growth factor binding protein 7
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_001553
• Gene symbol: IGFBP7
• Gene ID (NCBI): 3490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGFBP7, also named as MAC25, PSF, IBP-7 and TAF, binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. IGFBP7 stimulates cell adhesion.

Publikationen

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI.; Authors - Ali C M Johnson
human	IHC,WB	Cancer Lett; IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis.; Authors - Wei Dang
mouse	IF	Hum Genet; MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.; Authors - Sandeep Aryal
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma
rat	IHC	J Cell Biochem; IGFBP7 contributes to epithelial-mesenchymal transition of HPAEpiC cells in response to radiation; Authors - Yazhen Zhong
	WB	J Cell Mol Med; The Circ_35953 induced by the NF-κB mediated the septic AKI via targeting miR-7219-5p/HOOK3 and IGFBP7 axis; Authors - Yuqing Feng