IGHMBP2 Polyklonaler Antikörper

IGHMBP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 23945-1-AP

Synonyme

ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2

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  WB analysis using 23945-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 23945-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 23945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 23945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

23945-1-AP bindet in WB, IHC, ELISA IGHMBP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGHMBP2 fusion protein Ag21057
• Vollständiger Name: immunoglobulin mu binding protein 2
• Berechnetes Molekulargewicht: 993 aa, 109 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC105088
• Gene symbol: IGHMBP2
• Gene ID (NCBI): 3508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.

Publikationen

Species	Application	Title
human	WB	bioRxiv; IGHMBP2 deletion suppresses translation and activates the integrated stress response; Authors - Jesslyn E Park
Human	WB	Life Sci Alliance; IGHMBP2 deletion suppresses translation and activates the integrated stress response; Authors - Jesslyn Park