IGSF8/CD316 Polyklonaler Antikörper

IGSF8/CD316 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14387-1-AP

Synonyme

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14387-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14387-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14387-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14387-1-AP

  IP Result of anti-IGSF8 (IP:14387-1-AP, 3ug; Detection:14387-1-AP 1:500) with mouse brain tissue lysate 5000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14387-1-AP bindet in WB, IP, ELISA IGSF8/CD316 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGSF8/CD316 fusion protein Ag5846
• Vollständiger Name: immunoglobulin superfamily, member 8
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC053881
• Gene symbol: IGSF8
• Gene ID (NCBI): 93185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

Publikationen

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.; Authors - Ming-Jie Kuang
rat	WB	Biochem Biophys Res Commun; Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.; Authors - Bao-Cheng Yang