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IK Polyklonaler Antikörper

IK Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31833-1-PBS

Synonyme

RED, Protein RER, Protein Red, IK factor, IK Cytokine

Filter:
  • Western Blot (WB) analysis of various lysates using IK Polyclonal antibody (31833-1-AP)

    WB analysis using 31833-1-AP (same clone as 31833-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IK Polyclonal antibody (31833-1-AP)

    IHC staining of mouse testis using 31833-1-AP (same clone as 31833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IK Polyclonal antibody (31833-1-AP)

    IF Staining of A431 using 31833-1-AP (same clone as 31833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31833-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IK und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IK fusion protein Ag37095
Vollständiger Name IK cytokine, down-regulator of HLA II
Berechnetes Molekulargewicht 557 aa, 66 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC013005
Gene symbol IK
Gene ID (NCBI) 3550
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.