IKBKB Polyklonaler Antikörper

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• KD/KO Validated

IKBKB Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

79

Rezensionen (1)

Kat-Nr. : 15649-1-AP

Synonyme

I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 15649-1-AP

  WB result of IKBKB antibody (15649-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKBKB transfected Jurkat cells.

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  WB analysis of Jurkat using 15649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15649-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15649-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 72 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

15649-1-AP bindet in WB, IP, IHC, IF, ELISA IKBKB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IKBKB fusion protein Ag8191
• Vollständiger Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Berechnetes Molekulargewicht: 756aa,81 kDa; 256aa,29 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 86 kDa, 87 and 29 kDa
• GenBank-Zugangsnummer: BC006231
• Gene symbol: IKBKB
• Gene ID (NCBI): 3551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. This antibody can identify 4 isoform of IKBKB with the molecular weight of 80, 86, 87 and 29 kDa.

Publikationen

Species	Application	Title
mouse	WB	Drug Des Devel Ther; Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways; Authors - Li-Shan Yan
human	IF	Cell Death Discov; tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKB.; Authors - Dianbo Long
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
		Br J Pharmacol; PMID: 28963856; Authors - Xiao Han
mouse	WB	J Agric Food Chem; A Flower-like Brain Targeted Selenium Nanocluster Lowers the Chlorogenic Acid Dose for Ameliorating Cognitive Impairment in APP/PS1 Mice; Authors - Zhiwei Li
human	WB	Aging (Albany NY); HSP90 acts as a senomorphic target in senescent retinal pigmental epithelial cells.; Authors - Dan-Dan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Eugenia (Verified Customer) (03-16-2022)

Antibody worked well with specific band although a couple of non-specific bands also appeared.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549 lung cancer cell line