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IKKB Rekombinanter Antikörper

IKKB Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250538F11

Kat-Nr. : 81347-2-PBS

Synonyme

IKBKB, EC:2.7.11.1, EC:2.7.11.10, I kappa B kinase 2, I kappa B kinase beta

Filter:
  • Western Blot (WB) analysis of various lysates using IKKB Recombinant monoclonal antibody (81347-2-RR)

    WB analysis using 81347-2-RR (same clone as 81347-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81347-2-RR (IKKB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81347-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IKKB Recombinant monoclonal antibody (81347-2-RR)

    IF Staining of THP-1 using 81347-2-RR (same clone as 81347-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IKKB antibody (81347-2-RR, Clone: 250538F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81347-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81347-2-RR

    Affinity and Kinetic Characterization of 81347-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81347-2-RR against Human IKKB were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

81347-2-PBS bindet in WB, IF/ICC, Indirect ELISA IKKB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IKKB fusion protein Ag8191
Vollständiger Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Berechnetes Molekulargewicht756aa,81 kDa; 256aa,29 kDa
Beobachtetes Molekulargewicht80-85 kDa
GenBank-ZugangsnummerBC006231
Gene symbol IKBKB
Gene ID (NCBI) 3551
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.