IKBKG Monoklonaler Antikörper

IKBKG Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F2E2

Kat-Nr. : 66460-1-Ig

Synonyme

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

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  WB analysis using 66460-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66460-1-Ig (IKBKG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66460-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66460-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66460-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IKBKG antibody (66460-1-Ig, Clone: 1F2E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66460-1-Ig bindet in WB, IHC, IF/ICC, ELISA IKBKG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IKBKG fusion protein Ag13358
• Vollständiger Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC012114
• Gene symbol: IKBKG
• Gene ID (NCBI): 8517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.

Publikationen

Species	Application	Title
human	WB	Virol Sin; Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling; Authors - Jinfu Sun