IKBKG Monoklonaler Antikörper

IKBKG Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2E2

Kat-Nr. : 66460-1-PBS

Synonyme

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

---

• 

  WB analysis using 66460-1-Ig (same clone as 66460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66460-1-Ig (IKBKG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IKBKG antibody (66460-1-Ig, Clone: 1F2E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66460-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IKBKG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IKBKG fusion protein Ag13358
• Vollständiger Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC012114
• Gene symbol: IKBKG
• Gene ID (NCBI): 8517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.