IKIP Polyklonaler Antikörper

IKIP Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14589-1-AP

Synonyme

IKBIP, IKBKB interacting protein, IKIP, IKK interacting protein

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 14589-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14589-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14589-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 14589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14589-1-AP bindet in WB, IHC,ELISA IKIP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IKIP fusion protein Ag6119
• Vollständiger Name: IKK interacting protein
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC058933
• Gene symbol: IKIP
• Gene ID (NCBI): 121457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKIP(Inhibitor of nuclear factor kappa-B kinase-interacting protein) is also named as IKBIP and acts as a novel p53 target gene with proapoptotic function. A variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining. EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins(PMID:15389287).