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IKZF1 Monoklonaler Antikörper

IKZF1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1D6B5

Kat-Nr. : 66966-1-Ig

Synonyme

DNA binding protein Ikaros, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, Daudi-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Hausschwein-Thymusgewebe, Kaninchenmilzgewebe, Ramos-Zellen, Sp2/0-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66966-1-Ig bindet in WB, IF, CoIP, ELISA IKZF1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IKZF1 fusion protein Ag28637
Vollständiger Name IKAROS family zinc finger 1 (Ikaros)
Berechnetes Molekulargewicht 477 aa, 53 kDa
Beobachtetes Molekulargewicht 55-65 kDa
GenBank-ZugangsnummerBC018349
Gene symbol IKZF1
Gene ID (NCBI) 10320
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IKZF1 antibody 66966-1-IgProtokoll herunterladen
IHC protocol for IKZF1 antibody 66966-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanCoIP

Nat Commun

YTHDF2 upregulation and subcellular localization dictate CD8 T cell polyfunctionality in anti-tumor immunity

Authors - Haiyan Zhang
humanWB

Int J Mol Sci

Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2.

Authors - Elanora Dovat
humanWB

Cancer Res

Targeted Degradation of SOS1 Exhibits Potent Anticancer Activity and Overcomes Resistance in KRAS-Mutant Tumors and BCR-ABL-Positive Leukemia

Authors - Ziwei Luo
mouseIF

Cell Commun Signal

IKZF1 as a potential therapeutic target for dendritic cell-mediated immunotherapy in IgA nephropathy

Authors - Fei Peng