HELIOS Monoklonaler Antikörper

HELIOS Monoklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1F10

Kat-Nr. : 68558-1-Ig

Synonyme

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

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  WB analysis of Jurkat using 68558-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MOLT-4 using 68558-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 68558-1-Ig

  IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68558-1-Ig bindet in WB, IP, ELISA HELIOS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HELIOS fusion protein Ag4357
• Vollständiger Name: IKAROS family zinc finger 2 (Helios)
• Berechnetes Molekulargewicht: 500 aa, 57 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC028936
• Gene symbol: HELIOS
• Gene ID (NCBI): 22807
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).