IKZF3 Polyklonaler Antikörper

IKZF3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 19055-1-AP

Synonyme

AIO, AIOLOS, IKZF3, Zinc finger protein Aiolos, ZNFN1A3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 19055-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19055-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19055-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19055-1-AP

  Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

19055-1-AP bindet in WB, IHC, IF, ELISA IKZF3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IKZF3 fusion protein Ag5537
• Vollständiger Name: IKAROS family zinc finger 3 (Aiolos)
• Berechnetes Molekulargewicht: 509 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58-70 kDa
• GenBank-Zugangsnummer: BC032707
• Gene symbol: IKZF3
• Gene ID (NCBI): 22806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; IMiDs uniquely synergize with TKIs to upregulate apoptosis of Philadelphia chromosome-positive acute lymphoblastic leukemia cells expressing a dominant-negative IKZF1 isoform.; Authors - Daisuke Harama
mouse	WB	Eur J Med Chem; Discovery of novel BTK PROTACs with improved metabolic stability via linker rigidification strategy; Authors - Song Chen
human	WB,IHC	Sci Rep; Overexpression of Aiolos promotes epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer cells.; Authors - Jung-Jyh Hung