Aiolos/IKZF3 Polyklonaler Antikörper

Aiolos/IKZF3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19055-1-PBS

Synonyme

IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS

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  WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19055-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Aiolos/IKZF3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aiolos/IKZF3 fusion protein Ag5537
• Vollständiger Name: IKAROS family zinc finger 3 (Aiolos)
• Berechnetes Molekulargewicht: 509 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58-70 kDa
• GenBank-Zugangsnummer: BC032707
• Gene symbol: IKZF3
• Gene ID (NCBI): 22806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.