IKZF4 Rekombinanter Antikörper

IKZF4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240357D5

Kat-Nr. : 83471-5-RR

Synonyme

ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5

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  WB analysis of K-562 using 83471-5-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83471-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83471-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of U2OS using 83471-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83471-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83471-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA IKZF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IKZF4 fusion protein Ag34767
• Vollständiger Name: IKAROS family zinc finger 4 (Eos)
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC160169
• Gene symbol: IKZF4
• Gene ID (NCBI): 64375
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)