IL-1 beta Monoklonaler Antikörper

IL-1 beta Monoklonal Antikörper für FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A7C6

Kat-Nr. : 65276-1-PBS

Synonyme

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

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  FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 65276-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 65276-1-Ig against IL-1 beta were performed. The affinity constant is 102 nM.

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Geprüfte Anwendungen

Produktinformation

65276-1-PBS bindet in FC (Intra), Indirect ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein HZ-1164
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)