IL-1 beta Polyklonaler Antikörper

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• KD/KO Validated

IL-1 beta Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16806-1-PBS

Synonyme

Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta

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  WB analysis using 16806-1-AP (same clone as 16806-1-PBS)

  Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 16806-1-AP (same clone as 16806-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 16806-1-AP (same clone as 16806-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16806-1-AP (same clone as 16806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 16806-1-AP (same clone as 16806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using IL-1 beta antibody (16806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16806-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Ag10295
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.