IL-1 beta Monoklonaler Antikörper

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• KD/KO Validated

IL-1 beta Monoklonal Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1B4

Kat-Nr. : 66737-1-PBS

Synonyme

2A1B4, Catabolin, IL 1, IL 1 beta, IL1 beta

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  WB analysis using 66737-1-Ig (same clone as 66737-1-PBS)

  Untreated, LPS and Brefeldin A-treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66737-1-Ig (same clone as 66737-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 66737-1-Ig (same clone as 66737-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP80001-6

  Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-PBS. Detection antibody: 66737-1-PBS. Standard:HZ-1164. Range: 15.6-1000 pg/mL.

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  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using IL-1 beta antibody (66737-1-Ig, Clone: 2A1B4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66737-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66737-1-Ig against Human IL1B were performed. The affinity constant is 5.03 nM.

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Geprüfte Anwendungen

Produktinformation

66737-1-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Ag26030
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.