IL-1 beta Rekombinanter Antikörper

IL-1 beta Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240902D6

Kat-Nr. : 82696-15-RR

Synonyme

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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  WB analysis of THP-1 using 82696-15-RR

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82696-15-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 82696-15-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated THP-1 cells using IL-1 beta antibody (82696-15-RR, Clone: 240902D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82696-15-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-15-RR against Human IL-1 beta were performed. The affinity constant is 2.09 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LPS und Brefeldin-A behandelte THP-1-Zellen, A431-Zellen, HT-1080.Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: LPS und Brefeldin-A behandelte THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

82696-15-RR bindet in WB, IF/ICC, ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Publikationen

Species	Application	Title
human	WB	Food Sci Nutr; Resveratrol Promotes Wound Healing by Enhancing Angiogenesis via Inhibition of Ferroptosis; Authors - Yujie Pan