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IL-1 beta Rekombinanter Antikörper

IL-1 beta Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3I9

Kat-Nr. : 82696-6-PBS

Synonyme

3I9, Catabolin, IL 1, IL 1 beta, IL1 beta

Filter:
  • Western Blot (WB) analysis of THP-1 cells using IL-1 beta Recombinant antibody (82696-6-RR)

    WB analysis of THP-1 using 82696-6-RR (same clone as 82696-6-PBS)

    LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-6-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IL-1 beta Recombinant antibody (82696-6-RR)

    IF Staining of THP-1 using 82696-6-RR (same clone as 82696-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using IL-1 beta antibody (82696-6-RR, Clone: 3I9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82696-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82696-6-RR

    Affinity and Kinetic Characterization of 82696-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82696-6-RR against Human IL-1 Beta were performed. The affinity constant is 202 nM.

Geprüfte Anwendungen

Produktinformation

82696-6-PBS bindet in WB, IF/ICC, Indirect ELISA IL-1 beta und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-1 beta fusion protein Eg0286
Vollständiger Name interleukin 1, beta
Berechnetes Molekulargewicht 269 aa, 31 kDa
Beobachtetes Molekulargewicht30-35 kDa
GenBank-ZugangsnummerBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.