IL-1 alpha Rekombinanter Antikörper

IL-1 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240431G10

Kat-Nr. : 98052-1-RR

Synonyme

IL1-alpha, Il1a, IL-1,IL1, IL1 alpha, Il 1a

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  FC experiment of RAW 264.7 using 98052-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone:240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of RAW 264.7 using 98052-1-RR

  1x10^6 LPS treated (red) or untreated (black) RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone: 240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 98052-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98052-1-RR against Mouse IL-1 alpha were performed. The affinity constant is 0.495 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): mit LPS behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98052-1-RR bindet in FC (Intra) IL-1 alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 1 alpha
• Berechnetes Molekulargewicht: 31kd
• GenBank-Zugangsnummer: NM_010554
• Gene symbol: Il1a
• Gene ID (NCBI): 16175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).