IL-1 alpha Rekombinanter Antikörper

IL-1 alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

240431G10

Kat-Nr. : FITC-98052

Synonyme

Il1a, IL1-alpha, 240431G10, Il 1a, IL1 alpha

---

• 

  FC experiment of RAW 264.7 using FITC-98052

  1x10^6 untreated or LPS-treated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of RAW 264.7 using FITC-98052

  1x10^6 LPS-treated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10) (red) or 0.25 ug FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10) (black, dashed). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): mit LPS behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98052 bindet in FC (Intra) IL-1 alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1 alpha fusion protein Eg0263
• Vollständiger Name: interleukin 1 alpha
• Berechnetes Molekulargewicht: 31kd
• GenBank-Zugangsnummer: NM_010554
• Gene symbol: Il1a
• Gene ID (NCBI): 16175
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).